The aim of the course is to introduce modern molecular physiology and metabolism, which, thanks to new technologies (such as metabolomics and fluxomics), are beginning to complement other OMICs approaches in a wide range of biological applications. The course will introduce various topics in the physiology of unicellular organisms, plants and animals, including humans, and related biomedical problems in selected chapters. The aim is to introduce selected hot topics in modern molecular biochemistry and physiology and to demonstrate modern methodological approaches, not to cover the whole molecular physiology in a textbook fashion.